Fluorescent Proteins/lacZ System true CAG-GFP conditional transgenic mice (REP08 mice). The transgene consists of the CAG promoter and the EGFP cDNA separated by a floxed neo cassette. When the floxed neo cassette is excised by crossing with Cre mice, EGFP is expressed. The transgene was injected into D3 ES cells to generate mice having a single copy of the transgene. The level of GFP fluorescence is relatively low (Developer, personal communication). Masaru OKABE 国立大学法人大阪大学 <a href='https://brc.riken.jp/mus/pcr02096'>Genotyping protocol -PCR-</a> CAG-GFPコンディショナルトランスジェニックマウス (REP08マウス) 。creリコンビネース存在下で，loxP-neo-loxP配列が除かれるとCAGプロモーターの制御下にEGFPが発現する。挿入されているトランスジーンは1コピーのため、発現量は低く、蛍光強度は弱い (開発者情報) 。, Developed by Masato Ikawa, Faculty of Pharmaceutical Sciences, Osaka University in 2004. The transgene was injected into the D3 ES cells. The mutant mice were crossed to C57BL/6NCrSlc. CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, E. coli neo, pA, Jellyfish GFP cDNA Carrier x Carrier [C57BL/6NCrSlc] Carrier x Carrier [C57BL/6NCrSlc] REP-08 REP-08 RBRC02096 大阪大学薬学研究科・井川正人先生（2004）。D3 ES細胞へトランスジーンをインジェクションすることにより作出。 C (3-6 months) 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Biochem Biophys Res Commun., 321, 275-279 (2004).<br>非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること（https://www.ccb.osaka-u.ac.jp/en/）。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は寄託者と別途協議を行う。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。 Osaka Univ. Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>CAGGS MTA (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/CAGGS_MTA.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol><A HREF="https://egr.biken.osaka-u.ac.jp/achievement/bio_resources" target="_blank">Lab HP</A> CAG-GFPコンディショナルトランスジェニックマウス (REP08マウス) 。creリコンビネース存在下で，loxP-neo-loxP配列が除かれるとCAGプロモーターの制御下にEGFPが発現する。挿入されているトランスジーンは1コピーのため、発現量は低く、蛍光強度は弱い (開発者情報) 。 C（3〜6か月） 岡部　勝 B6.Cg-Tg(CAG-floxed Neo-EGFP)REP08Osb B6.Cg-Tg(CAG-floxed Neo-EGFP)REP08Osb General Purpose The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem Biophys Res Commun., 321, 275-279 (2004).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the BIOLOGICAL RESOURCES. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. Cre/loxP system